FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_215_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14175669
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAT17450.012.5630941
GTCCGTT16550.011.3539571
GTCCGAT14850.011.2477661
GTCCGGG26950.010.975181
GTCCGCT19800.010.7205271
CCCGAAT17700.010.4196731
CCCGTAT13050.010.3993351
GTCGCTT19100.010.3846761
GTCGAAT13000.010.1716581
GTCGGTT13500.010.05269051
CGCGTAA5856.9048838E-910.02668667
CTCGTAT20550.09.821271
GATCGCG6001.0490112E-89.77401840
GTCGCAT10400.09.7032261
TCGACCG9250.09.69618631
CGATCGG9300.09.64419341
GTCCGGT20200.09.4746421
TCGCGTA5857.370181E-89.43694766
GTCGGAA20350.09.4048031
GTCGGCT23600.09.2892371