FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_214_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_214_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5186256
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT6150.011.77074449
TCTCGAT7605.456968E-129.97858248
TTCGCTA2800.00335574489.8483267
GCGTCGT6151.3833414E-89.60950619
ATTCCGT7606.366463E-119.52583753
GATTCCG7951.546141E-109.10637252
TTCTCGA8152.5465852E-108.88110547
TCGATTC8607.3123374E-108.416882550
TCACCCG9152.8376235E-108.2867033
CGGGGTA5908.024574E-68.1789661
GGCGGTA8657.230483E-97.969743359
CGGTACA8901.22818165E-87.745724761
CGATTCC9656.888513E-97.501996551
GAATCGT7959.894356E-77.36990644
CGTGGGT11051.6971171E-97.174917757
GTTGAAT20500.07.0676941
ACGGGCG11252.4701876E-97.047363856
GCGGTAC10302.42253E-87.02762160
GTCTAAG9352.381039E-77.004558644
TACATCG11051.2829332E-86.86183933