FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5186256
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA1451.1745069E-519.2011051
TCGACCG3501.8189894E-1216.75518831
CGATCGG4350.015.86006641
CGACCGT3857.2759576E-1215.2319932
GTTCGAC4152.3646862E-1114.13033729
GTCGGTT3659.587893E-913.3487151
GCGATCG5004.0017767E-1112.41855140
CGTGCGA4752.2009772E-1012.34545137
GTCGCAA2852.155669E-512.211231
GTCCGTT7200.012.084031
TCGACGC5156.548362E-1112.05649843
ATTCGAA5259.276846E-1111.8259378
CCGGTAT6800.011.7712661
CTATCGT2651.6071883E-411.7179874
AGTTCGA5351.2914825E-1011.60545128
TTCGACC5451.7826096E-1011.39272630
GTCGACT4601.9623258E-811.3484811
GTCGGAT6451.8189894E-1211.3308851
GGCGTTT7150.011.195051
GTCCGAT6551.8189894E-1211.1578951