FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_213_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_213_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10827187
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA17200.010.01965961
CTCGATT13050.08.98106849
GGCGGTA18550.08.91917759
GTCTAAG19600.08.79345344
GCGGTAC20300.08.31996460
GTCGAAT5857.1027243E-68.25396251
TCGATTC16650.07.86703150
GTCGGAT9709.1858965E-107.82242251
ATCGACT16600.07.68229636
CGAACTA8656.105438E-87.570333
TCTCGAT16450.07.543904348
TCACTAA33650.07.47744568
CTCACTA30250.07.40623337
ATTCCGT16300.07.402358553
ACTAGGC13651.8189894E-127.323097711
AATCCGA14750.07.243815
GATTCCG17150.07.23649152
GTTTAAT48150.07.1629851
CGAAAAT15000.07.12788631
ACTCACT40800.07.09604746