FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_213_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10827187
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG6750.016.35413431
CGACCGT7350.015.01910232
GTCCGTT13750.012.902941
CGCTAGT3356.602131E-712.4611361
GTTCGAC9200.012.37312429
GTCCGAT15900.011.8145681
GTCGTAT11050.011.6482481
GTCCGCT13300.011.5085931
GTTCCGT12100.011.4999471
TCGACGC10550.011.11710743
GTCGGTT8600.010.9216061
GTCGTTT19500.010.8821931
CGATCGG7950.010.84791341
TTCGACC10700.010.63857530
TCGCGTA4956.554728E-810.4555266
GTCGAGA9000.010.4362011
AAGTTCG11950.010.39163427
GTCCGGA12400.010.3800931
GTCGGAT15150.010.3328721
GTCCGAA10850.010.2598591