FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4539664
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGA4201.1122442E-59.8471564
CGGTACA6554.4074113E-99.47214561
TCGAGAT7151.8234277E-78.19475657
GGCGGTA8657.228664E-97.96967459
ACTTGCG3950.00624966767.85357410
AATCGGC4600.00290155977.493556546
AAATCGA8001.0925032E-67.32382254
GTCTAAG9201.7949242E-77.118844
GCGGTAC8806.499631E-77.05044560
TCACCCG8452.5875452E-66.9337963
CTCGATT5150.0086944686.69334749
AATCGAA6200.00102987126.67080075
TAGCATA13453.255991E-106.66290149
CCGGAAT5200.0094683726.6343971
GGAATCG6854.446387E-46.54081153
GAAATCG9552.508692E-66.4960293
ACACACG11201.19038305E-76.463762352
GTTAAAT23500.06.45936161
AGTATAC8107.4866155E-56.3825565
GTTCGAC6000.0053473936.31949644