FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_212_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4539664
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTA2551.366061E-917.5908472
GTCGTAA3251.888111E-914.981771
GTCCGGG9650.012.974591
GTCGCAA2204.148865E-412.646951
GTCGGTC6600.012.6469491
GTCGGTT3601.16086085E-712.5591231
CCGGCTA2100.00446999611.49828737
GTCGCAT3953.875357E-711.446291
GTCGAAT4055.354632E-711.1636651
GTCGATA2500.0012136411.1293151
CTCGGTT6603.6379788E-1211.066081
GTCGTCT7650.010.9110941
GTCCGAT3207.079741E-510.8684721
GCCGTAT4208.559764E-710.7649631
CTCGATT6553.092282E-1110.61957551
GCCCGTT8350.010.4129081
CCGGTAT4056.4766864E-610.3049211
CGACGTA2700.00246303510.2227274
GCCGTAA4107.4966156E-610.1792521
GTTCCGT5502.508932E-810.1175591