FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4731557
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT5650.014.03347449
TCGATTC6650.012.44131450
TTCTCGA6650.011.9221747
GATTCCG6700.011.83382352
TCTCGAT6900.011.491178548
CGATTCC8400.011.08124751
ATTCCGT8202.910383E-119.24870453
GTCTAAG9355.456968E-128.847744
CGTGGGT10550.08.82186557
GGCGGTA8001.6752892E-98.61747359
CCGGACA8851.4188117E-108.56860512
CGGTACA8353.741661E-98.25608761
CGTCGTA4050.00780481157.6611151
CGGACAC11951.6370905E-117.5643313
TCAATCC9656.9248927E-97.49995143
TTTCTCG10152.3228495E-97.471701646
GTCAATC7959.865162E-77.37123162
GCGGTAC9403.3702236E-87.33394260
ACCCGGC11951.5825208E-107.2104365
GTTCAAT13405.456968E-127.2070061