FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_294_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_294_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12226875
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG3401.4252376E-410.1436147
CGGTACA18500.08.95281561
GCGTAGT9559.094947E-128.66834818
GGCGGTA19800.08.19049959
CTCGATT15050.08.0230949
TTGCGTA11052.5465852E-117.80376916
GTCTAAG21850.07.736461644
GATTCCG16050.07.308171352
TCTCGAT15600.07.298105748
GTTTATT72500.07.202341
TTCTCGA16900.07.14491147
GTAGTCG9352.3418033E-77.011554757
GCGGTAC23800.06.95912160
CGCGGTA6007.0329546E-46.8985617
ATCGACT19300.06.7904956
GTTTTAT90700.06.7865251
CGCTAGA7201.10767476E-46.70816866
GTCGATT7753.8784827E-56.69305321
TAGTCGC11352.1396772E-86.68812758
GTTTAAT59500.06.6836791