FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_294_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12226875
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG5700.020.57728831
CGACCGT7400.016.31625232
GTTCGAC7950.014.319365529
GTCGCGA2551.08339984E-412.2247341
TCGACGC10200.012.17504143
GTCCAGT58800.011.5455841
TTCGACC10400.011.27793630
TCTCGAC10500.011.17093941
CCCTAGT9750.011.012711
CCCGTAT9650.010.7679011
AGTTCGA11550.010.751528
GTCGGAT16150.010.2945141
GTCGCTT17350.09.9817721
GTCCGAA12250.09.89621451
GTCCGTT14750.09.8626681
GTCGGAA20400.09.6779151
GTCGGTT11250.09.5443491
GTCGCTA10600.09.4760911
GTTCGGA12500.09.4211961
GTCGTCT23050.09.3166091