FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_293_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_293_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13617262
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT16150.011.96231649
TCTCGAT17450.011.66436548
GATTCCG20150.010.95708752
TCGATTC21450.010.29302150
ATTCCGT21300.09.71766353
TTCTCGA20000.09.65949847
CGGTACA20600.08.37501261
CGATTCC26600.08.17050851
GGCGGTA23250.08.16230659
TCACCCG28400.08.0152463
CCGGACA27650.07.609307312
GTTTAAT61850.07.380621
GTCGAAA7503.1053387E-67.37763831
TTTCTCG28350.07.057393646
CCCGGAC32400.07.025787411
GCGCTAG8402.3270131E-66.981190732
GTCTAAG26800.06.95100444
GTTTATT82250.06.8955131
ACTAGGC13656.366463E-116.822242711
CGCTAGT8201.2150378E-56.73093833