FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13617262
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG8450.020.8205831
CGACCGT9750.018.75212932
GTTCGAC12100.015.39500229
TCGACGC14050.012.76718643
TTCGACC14750.012.62935330
GTCCGCT18250.012.5258891
GTCGGTT13400.012.40691951
AGTTCGA15650.011.68203528
TCGCGTA6650.011.41324366
AAGTTCG16100.011.14126527
GTCCGAT15250.011.1289391
GTCCGTT14800.010.9992641
CGATCGG9650.010.724997541
TCTCGAC17400.010.70650741
GTCCGGT22400.010.669121
GTCGCTT19300.09.87035751
CGCGTAA7353.274181E-119.85689267
GCCGTTT29850.09.7468091
GCGATCG11350.09.72708940
GACCGTC19750.09.60671633