FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_292_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_292_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17526731
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT21250.08.92884949
GATTCCG23650.08.89778152
TCGATTC26200.08.29511350
CGCGGTA8901.4151738E-98.13875117
ATTCCGT24900.07.75839253
TCTCGAT24250.07.682077448
TTCTCGA25600.07.411620647
CGGTACA26350.07.33296661
GTCTAAG29400.07.15765144
GCGTATA10302.3823304E-87.034178739
GGCGGTA27400.06.925694559
TCACCCG33200.06.8565533
TTTCTCG34300.06.43654546
CGATTCC33350.06.4132651
GCGGTAC30800.06.385522460
CGGTAAT11005.826005E-76.27138319
GGAAAAT164850.06.14691731
CCGGACA33350.06.10189712
GTTTAAT80100.06.04469251
GTTCGAT20600.06.04385381