FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17526731
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG10800.012.45701531
CGACCGT11550.011.94678732
GTTCGAC12150.011.07271229
GTCCGAA18400.010.7301761
GTCCGTT22150.010.6337141
CGATCGG10650.010.04184941
CCCGAAT21050.010.0375451
GTCGGTT17150.09.6945321
CCCGTAT16450.09.6859391
GCCCTAT30850.09.5436291
GTCCGAT18550.09.5230491
GTCGTAT18300.09.4638681
CCGGTAT16500.09.4466621
CTCGTAT23950.09.4006441
GTCGGAT19650.09.3425011
GTCCGCT22700.09.3079441
GTCCTAT37350.09.273831
GTCCGTA20200.09.2595991
GGCGTTT29450.09.0563921
GTCGAAT13850.09.0033171