FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4509795
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA6453.2922435E-78.55854861
CTCGATT5806.302549E-68.3274349
TCGAGCC6807.384551E-78.1155157
TCTCGAT6858.215047E-78.058648
CAATCTA9001.7589628E-98.0479744
GTTTGAT25400.07.89487841
CTTTAAT25150.07.69841341
TTCTCGA6402.3465776E-57.54681747
CTTAGAT12102.5465852E-117.42915061
GAATCGA4700.00355921137.33857874
ATTCCGT6603.52963E-57.317800553
GTTCAAT11605.857146E-107.1532671
CGTGAAT4850.0047224167.1286851
GATTCCG6351.7390493E-47.06262452
AATCGAA5400.0017590767.0260095
TCGATTC6552.5442743E-46.84697250
TGCGCGT5050.00715300676.82996379
GGCGGTA6602.788997E-46.795778859
AATCCGG5600.00258104956.775085
GGGAAAT30250.06.74338251