FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4509795
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG1350.003244929715.33338840
GTCCGCT6450.015.0318031
CCGGTAT4456.91216E-1113.2282291
CCCGTAT3902.5982445E-812.4301451
GTCGTAT5753.6379788E-1212.0441161
GTCCGAA4359.249561E-911.9402871
GTCGAAA2953.231007E-511.7379091
CGTTCTA3551.4710331E-611.6611074
GTTCGAC2100.0044682311.49889429
GGCGTTT7550.011.4658391
CGGGTAA4853.776222E-911.4232851
GTCGAAT4602.1138476E-811.2913591
GTTCGAT5653.1468517E-1011.0315571
GTCGATT4451.6208833E-710.8938351
GCCGTAT4652.9699913E-710.4252831
GTCCGTA5452.330853E-810.1656751
GTCAGAT17450.09.9217281
GCCTGAT13650.09.8933811
GTTCCGT5603.5506673E-89.8933811
GTCGACA3501.8332334E-49.893381