FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_290_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_290_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15722295
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG29550.08.0533713
CTCGATT18450.07.479335349
GATTCCG20000.06.89955552
ATCGACT24700.06.84203436
TCTCGAT20450.06.74798948
TCGACTG22350.06.6355737
GTCTAAG26650.06.601951644
GCGTCGT20450.06.578472619
CGGTACA23100.06.5720561
TCGATTC22050.06.57100550
AATCGTC23400.06.48515225
GTAGTCG15009.094947E-116.44044557
GTTAAAT73600.06.43667461
CGGGAAT26500.06.39394661
GTTTAAT70250.06.25138431
GGAATCG29400.06.21748353
TTCTCGA23150.06.109951547
GTGAAAT116750.06.07177641
GTTTATT97800.06.046111
GTTTTTT208700.06.0311171