FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_289_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_289_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7402081
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT9250.09.69706649
GATTCCG10650.08.74621152
TCGATTC10601.8189894E-128.46200650
TCTCGAT9102.4738256E-108.34051748
TTCTCGA9951.8189894E-107.97468547
CGAAAAT10253.255991E-107.7599471
CGGAAAT9808.736606E-97.41050861
CGATTCC13203.6379788E-127.31795851
ATTCCGT12102.055458E-107.127881553
GTTTGAT43300.06.94842151
TTGCGTA6108.4923866E-46.78516516
GAATCGT10704.9612936E-86.76899154
GTTTTAT57500.06.73604441
GTTTATT45100.06.67110061
GACGTAA5700.003100726.657916541
CCGGACA14705.4569682E-116.569674512
AATCGTC8956.286895E-66.55110175
GTTTTTT96400.06.49316361
GTTAAAT35200.06.3859491
TCACCCG14754.0927262E-106.31326963