FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7402081
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT6700.014.475531
GTCCGTT8950.013.1585211
GTCCGCT9150.012.8709041
GCCGATT7350.012.2528531
GTCGGGT11800.012.03519251
GTCGGAT11950.011.5942671
GGCGTTT10550.011.4912381
CCGGCTA5502.0918378E-1011.28970158
GTCGTTT13000.011.1906981
GTCCGAT8500.011.0026191
GTCCGGT12950.010.9664321
GTTCGGA7300.010.9133021
GTCGGGA11850.010.8152011
CTCCTAT20400.010.696991
CGCTAGT2650.002041088710.4567171
TCGACCG3301.0511446E-410.45342731
GTCGGCT10850.010.2157771
GTCGAAT7800.010.2137321
GTCGTAA7503.6379788E-1210.1604431
GTCGGTA10250.010.1379151