FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_280_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_280_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18622792
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT23300.010.36403549
CGGTACA32000.010.13541661
GGCGGTA31950.09.82698359
GCGGTAC34450.09.31445560
TCTCGAT26550.09.225450548
GATTCCG28250.09.03637352
TCGATTC29550.08.98905850
GTCTAAG34900.08.89618644
TTCTCGA28550.08.69992447
ATTCCGT30500.08.48286153
TTTCTCG36200.07.718810646
GTTTAAT84950.07.36863141
TCACCCG37850.07.28979353
TAGCATA51650.07.2785719
CGATTCC38300.07.205632751
ACGGGCG47300.07.148495756
TAGGCGC6301.5791168E-47.118555547
CTAAGTA49650.07.086875446
GGGCGGT48450.07.050032658
CCCGGAC38800.06.93363511