FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_280_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18622792
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT23100.012.7439791
GTCGGTT17800.012.6471151
GTCCGCT24150.011.7596621
GTCGCAT14900.011.6220271
CCCGAAT19600.011.4856451
TCGACCG12150.011.07282431
GTCCGAA18750.010.8980521
CGACCGT12900.010.696465532
GTCCGAT18000.010.58251
TCGCGTA7201.8189894E-1210.54121166
CTCGTAT27000.010.5183641
GTCGAAT15100.010.3212821
CGCGTAA7403.6379788E-1210.25631467
GTCGGAA26000.010.1236781
GTCGCTT27750.09.9844721
CCCGTAT18250.09.8682151
GCGATCG13450.09.74765540
GTCGGTA17550.09.669791
GTCGTGT24650.09.5541081
GTCGAGA11650.09.5131031