FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4131643
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA6650.014.00941561
GCGGTAC7500.011.96161760
GTCTAAG7950.011.71587144
GGCGGTA7200.011.50127759
CTCGATT6252.0379957E-89.38379349
TCTCGAT6302.3193934E-89.30954448
GTCGTAT3000.0057593789.224141
ACACTCG3400.00159799029.13215238
CCGGTAA3100.00771986278.9008748
GTCGGTC4007.05652E-48.6476321
GGGCGGT10400.08.62595858
TCTAAGT10850.08.58444145
ATTCCGT7453.9153747E-88.33528553
CTCGAAT3750.00379340548.3017251
CCCGGTA4250.00132451498.1155027
TTCTCGA6858.223087E-78.05802247
ACACGGG11255.456968E-127.972867554
CATATGC12200.07.916324612
TCGATTC7001.1406883E-67.885444650
TCACCCG8901.2129021E-87.75076153