FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4131643
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT3800.016.4087491
GTCCGTT5100.014.9430431
GTCCGGT5950.014.5549131
GCCGTAT3608.431016E-913.471381
GTCGGCT6200.013.40931
GTCGTAT4508.367351E-1113.0864831
GTCCGTA5003.6379788E-1112.4706491
TCGACCG2551.1248748E-412.17522531
GTCGGAG9500.012.0330821
CCCGTAT3752.079978E-712.0087731
CGATCGG2902.8171684E-511.89619841
GTCGCAA2357.473248E-411.7925761
GTCGCAT3256.702552E-611.7245421
GTCCGAA4451.2872988E-811.6766371
CGTCGCG2701.9159135E-411.4985473
GTCGGTA4258.533607E-811.4110521
TCCGCTA2150.005294631211.2401152
GTCCAGT19500.011.1916081
TCGGTTA2802.665209E-411.0967462
GTCGTTT7550.011.011611