FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13452777
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCG18800.010.4595552
CTCGATT16100.010.28543549
TCTCGAT18900.08.94436848
TCGATTC20700.08.83286450
TTCTCGA20250.08.68871747
GGCGGTA23200.08.62572859
ATTCCGT19700.08.58076153
CGGTACA23400.08.55206761
GTCTAAG25700.08.456717544
ACTAGGC16400.08.20215411
CGATTCC24800.07.650802651
GCGGTAC28000.07.2703160
AATCGGC16350.06.962776746
ATCGCGA5956.391534E-46.95665619
TCACCCG27800.06.94759133
ACACACG34100.06.87939952
GGGCGGT32900.06.71181258
GTTTAAT60050.06.68063351
CCCGGAC27900.06.675698811
TCGTTGA18650.06.659162552