FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13452777
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG6150.015.144917531
GTTCGAC8350.012.39399829
CGACCGT7650.012.17532532
GTCCGAA12550.012.1432271
GTCCGCT15850.011.5816841
GTCCGTT15850.010.9261181
GTCGGTT10200.010.8661321
GTCGAGA11200.010.8236861
GCCCGTT23550.010.295141
CGCGTAT2700.002385497610.2624581
GTCGCTT18350.010.192551
CCGGCTA9800.09.85742537
GTCGCAA8500.09.7795181
GTCCGAT16800.09.6897751
GTCGGCT19800.09.4461261
TCGACGC10800.09.26284643
GTCCAGT63700.09.1890881
GCCCTAT23900.08.9850181
GTCGGAT18200.08.9444081
GTCCGGA17850.08.9257511