FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3648268
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT4702.4447218E-911.74417249
TCTCGAT5158.440111E-1011.38785648
CGATACG3105.56468E-511.12870329
ATTCCGT5958.240022E-1010.436522553
TTCTCGA5806.004484E-910.11163147
GATTCCG6151.4624675E-910.09712452
CCGGACA6901.0004442E-109.99709912
TCACCCG7605.456968E-129.9844933
CCCGGAC8353.6379788E-129.50010811
CGTCATA3450.00182449329.00010436
TTTCTCG8102.0845619E-98.51756646
TCGATTC7353.1126547E-88.44861450
CGTGGGT8252.926754E-98.36476557
CGATTCC7504.3854016E-88.27964151
TCGAGCG4603.176906E-48.24749413
TACGATA4250.00131670918.12030127
CGGTACA5101.0759447E-48.11896561
CGGCCCG11255.456968E-127.97102078
GCCCGGA10851.6370905E-117.947003410
CGAAAAT4855.4574147E-47.84257361