FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3648268
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA952.9878886E-421.8742711
TCGACCG2303.45608E-1019.49834631
CGACCGT2851.6370905E-1118.15635532
GTCGAAT2351.7417551E-716.211781
TCGACGC3251.2369128E-1015.9212943
GTTCGAC3151.3624231E-915.33161329
GTCGCAT2756.2791514E-815.1131321
TCTCGAC3657.1304385E-1014.17785141
TACCGCG1550.00811434313.35498736
GTCGTTT6550.013.2191841
GTTCGAT7000.012.8641561
GTCCCGT6350.012.5446941
GTCCGTT5156.184564E-1112.1051791
TTCGACC3752.1950655E-711.95898630
CGGATTA2601.3477066E-411.94145864
GCCCGAT4702.2973836E-911.7903861
TAGTTCG2651.6078507E-411.71727240
CGAATTT3601.7413931E-611.4987093
GTTTCGA4853.763489E-911.4257361
CCGGTAT3651.9592353E-611.3866061