FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_276_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_276_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16769306
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA8001.5077603E-88.192327517
GATTCCG21450.07.880643452
CTCGATT21850.07.4207649
GGCGGTA23850.07.233238759
TCTCGAT21500.07.220774748
TCGATTC23600.07.16270450
CGGTACA25600.07.008623661
GTTTAAT80900.06.88096481
CGTAACG11051.26201485E-86.867913730
TTCTCGA22600.06.716569447
TAAATCG12908.785719E-106.6842083
GTCTAAG29050.06.531544744
GGAAAAT163350.06.4981721
GACGTAA12906.1099854E-96.418485641
GCGGTAC27950.06.29589860
GTTAAAT82350.06.2139831
ATTCCGT25650.06.186761453
TCAATCG18401.8189894E-126.18579863
GTTTATT107800.06.1261351
GTCAATC30600.06.09417532