FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_275_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_275_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12814519
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT16650.014.71070949
TCTCGAT17600.014.11289448
TCGATTC18350.013.34765650
GATTCCG18450.013.08833452
TTCTCGA20050.012.56009547
ATTCCGT20300.012.23542853
TCACCCG25450.011.1127713
CGGTACA21500.010.431313561
GGCGGTA20850.010.09433459
CCCGGAC26700.010.07607711
CGATTCC24750.09.89614151
ACCCGGC32550.09.2184465
CGTGGGT30800.09.185801557
TTTCTCG27800.09.18225446
CCGGACA27250.09.11351712
GCGGTAC24500.08.87234860
CGGCCCG33950.08.7367498
GTCTAAG25500.08.52284844
GTTTAAT62100.08.2982331
GCCCGGA35550.07.66446910