FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12814519
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA2859.730502E-814.5831651
TCGACCG9250.014.54435331
CGACCGT9350.014.01985532
GTCGGTT11900.013.3883261
GTTCGAC10350.012.99852629
GTCGTAT14400.011.5450051
TCGCGTA6451.8189894E-1211.23207366
TCTCGAC13850.011.20916541
CCCGTAT12100.011.1633531
GTCCGTT16200.011.1174121
TTCGACC12200.011.02748130
GTCCGAT13500.010.7753381
GTCGTTT23600.010.5666151
GTCGAAT10850.010.5341531
TCGACGC13150.010.23065943
CGCGTAA6756.548362E-1110.2217867
GTCCGCT15300.010.18676951
GTCCGAA12700.010.0905161
CTCGTAT17650.010.0078521
GATCGCG5201.3484714E-79.95230840