FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_274_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_274_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3697984
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA6000.013.80089661
GTCTAAG7350.012.672749544
GGCGGTA7550.010.967154559
GCGGTAC7900.010.48169360
ACGGGCG9350.09.9628156
ACACACG9550.09.75297652
TAGCATA9700.09.6007289
GGGCGGT9900.09.40931958
GATTCCG5553.480909E-79.32341752
CTAAGTA11100.09.01239246
CTCGATT3450.00182586518.99936749
ACACGGG10053.6379788E-128.58136954
GTTCGAT5301.783184E-58.4811691
CGAAAAT5402.2553182E-58.324111
TGTCTAA12850.08.32248443
GCATATG12100.08.26678311
TCTAAGT11950.08.08311345
CATATGC11203.6379788E-128.00728212
ATTCCGT6051.1104525E-57.98269353
TAAGTAC11255.456968E-127.97255747