FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3697984
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG2156.6118446E-817.6492231
GTCCGTT4800.016.5899961
CGACCGT2705.265065E-815.33164432
GTCGTTT6000.014.42608451
CGCGAGT1709.130835E-414.20412543
CGTATCA2506.1220235E-613.7988543
CGAATCT4457.4578566E-1113.1786813
TCGACGC3153.4626282E-713.141232543
GTTCGAC3204.1920975E-712.93607529
CCCGATT3002.7566293E-612.6949551
GTCGGGT5003.6379788E-1112.4641371
GTTCGGA3103.978739E-612.285441
CCCGAAT4802.4738256E-1012.2621721
GTCGCGT2306.2653027E-412.04264451
AAGTTCG3451.0427775E-611.99835427
CCGGCAT4501.5303158E-811.5408681
CCGAATC4501.6010745E-811.5055792
GTCGTAT3359.444015E-611.3686161
GCCTAAT3702.3167213E-611.2289531
TTCGCTA3702.4035362E-611.1946172