FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20550447
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG12100.017.39081631
CGACCGT13500.015.842854532
GTTCGAC16600.013.09186929
TCGACGC19050.011.9514143
GTCCGTT26000.011.8558261
GTCGCAT16200.011.5451
TTCGACC19750.011.35326430
AGTTCGA21250.011.03840828
GTCGGAT31800.010.8915091
CCCGAAT26850.010.8355291
AAGTTCG20750.010.47318827
GTCGGTT17050.010.3600291
GTCCGCT25450.010.2067791
GTCGTTT34450.010.1542371
CCGGTAT23150.09.874341
GTCGCTT27500.09.8237461
GTCCGGT34350.09.7804791
GTCCAGT96700.09.7422121
CCCGTAT16800.09.6895531
GGCGTTT33200.09.597651