FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_250_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_250_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15724788
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT19550.09.70549649
CGGTACA24600.09.25737761
GATTCCG21550.08.96469752
ATTCCGT23000.08.84950553
TCACCCG29300.08.7104973
TCTCGAT21650.08.60481948
TCGATTC23750.08.27953850
GGCGGTA26250.08.01809859
CCGCTTA12750.07.846620653
GTCTAAG29450.07.73116944
TTCTCGA23850.07.66633647
GCGGTAC29800.07.526208460
ACACACG36750.07.040423452
CGATTCC27350.06.937455751
TCACTAA46050.06.66559938
ACTAGGC17600.06.66291711
CGCTAGT6955.246732E-46.4525933
TTTCTCG32550.06.358808546
CCGGACA31600.06.33075712
TACATCG29550.06.3025333