FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_250_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15724788
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG9200.017.99814431
CGACCGT10100.015.71125132
GTTCGAC11850.013.97319829
TCGACGC13400.013.12886643
CGCTAGT5359.094947E-1212.3005521
GTCGGTT12850.011.8597171
CCCGAAT18400.011.8589851
CCCGTAT12600.011.5452561
GTCGTAT15300.011.3188771
TTCGACC16100.010.71318130
GCCGTAT15250.010.6746291
AGTTCGA15950.010.59711428
TCTCGAC16600.010.3913241
CCCGATT14300.09.9305341
GTCCGAT18900.09.7126751
GTCCGAA16100.09.6808041
GTCCGGA19550.09.5669121
GTCGTTT27600.09.5373841
CGCGTAT3652.7899642E-49.4892511
GTCCAGT70050.09.4438711