FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_249_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_249_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8420795
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTCG7555.456968E-1210.05398557
CGGTACA11800.09.94184961
CTCGATT10450.08.583570549
TCTCGAT11400.08.47357548
GCGGTAC14100.08.32012860
ATTCCGT12050.08.3025553
TAGTCGC10052.5465852E-118.2396358
GATTCCG11003.6379788E-128.15419852
ACTAGGC9801.3278623E-108.09515111
GGCGGTA13400.07.98214159
GTCTAAG14900.07.640341844
CCGCTTA6907.864242E-67.49968153
GTTTATT54850.07.31084541
CGATACG8051.1857028E-67.2862729
TACGTCG6201.2954338E-47.233734636
AGTCGCC11555.5842975E-107.169547659
CGGGAAT15050.07.1205161
CGATCGA4900.0053298287.040641367
TACATCG16700.07.02210953
TTCTCGA11859.786163E-106.98704447