FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_249_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8420795
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG3900.020.3443431
GTCCGCT10300.017.481111
CGACCGT4700.016.14749532
GTTCGAC5000.015.86848929
TCGACGC6150.013.46197543
CGCGTAT1550.00792278213.403631
CCCGAAT11250.012.0036961
TCTCGAC7550.011.42336541
AAGTTCG7400.011.18779327
AGTTCGA7500.011.03862328
GTCGGAT9450.010.9923951
TTCGACC7450.010.65005730
GTCCGAT10800.010.5801811
CGGCTAT6959.094947E-1210.4625471
GTCGTTT15300.010.4104461
CGATCGG4703.6216807E-710.27616841
GTCCGCA13000.010.121461
CCCGTAT7655.456968E-129.9578181
GTCCGAA9100.09.8931561
CTCGACG9800.09.85620542