FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_248_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_248_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14529743
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT15950.08.0028949
CGCGGTA6251.7164974E-57.72677917
GTTTAAT69850.07.72279171
TCTCGAT18900.07.301489448
GTTTGAT85050.07.27771141
TCGATTC19150.07.02572550
GATTCCG19200.07.007428652
GTTTTTT191250.06.72601131
ATTCCGT22900.06.62845853
CGGTAAT8502.064744E-56.49300219
TTCTCGA20750.06.48420647
GTCAAAT48000.06.4115771
CGGTACA19400.06.40272561
GGAAAAT141800.06.34034971
GGCGGTA19050.06.339174359
GCGTATA7157.38634E-46.27290239
AATCGTC16301.10958354E-106.13603165
GTTGAAT54500.06.09103161
CGATTCC25550.06.075988351
TTACGCG6250.0081042756.071542355