FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14529743
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG6600.013.58972131
CGACCGT7200.012.45724532
GTCGTAA12250.011.5917651
GTCGGAT17450.011.5115661
GTCCGCT18700.011.4829091
GTCGATT14500.011.4650261
GTTCGAC7950.011.28187729
GTCCGTT18850.011.0240641
GTCGCAT12300.010.6999151
GTCGAAT12750.010.5939091
CCCGTAT12450.010.5711
CTCGTAT21500.09.987461
GTCGCTT20600.09.9194281
GTCGGTT13600.09.6771291
TCGACGC10350.09.66545643
GTCGCAA7901.2732926E-119.6448941
GTCCGAT14200.09.5121361
GTCGTTA10800.09.2998531
GTCCGAA15300.09.2809891
GTCGCGA3000.0057017149.2357161