FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7029931
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT8600.014.03965549
GATTCCG10900.012.97610152
TCTCGAT9800.012.6726248
TCGATTC10400.012.27312850
GTCTAAG12400.010.29337144
GGCGGTA12450.010.25319959
TTCTCGA11450.010.24378147
CGGTACA12250.010.13910561
ATTCCGT12550.09.89567953
GCGGTAC13350.09.30367460
TCACCCG14350.09.1337813
CGATTCC14750.09.12135751
CCGGACA14100.08.56246312
CCCGGAC15000.08.50833911
CGTGGGT15650.07.936247357
TAAATCG4855.607909E-47.8229373
TTTCTCG15050.07.56384546
ACCCGGC17450.07.51100065
CGTAACG5200.00117794817.29717230
GCGTAAC5754.3081757E-47.200592529