FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7029931
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG4400.015.68025731
CGACCGT4950.013.93800632
GTCCGTT9050.013.7738251
GTCCGCT9150.013.6232911
GTTCGAC5100.013.52806529
GTCGCAT4601.200533E-1012.7965151
GTTCGAT8200.012.2457321
GTCGCAA3408.3546E-712.2208931
CGATCGG5853.6379788E-1211.794620541
TTCGACG2050.003731087311.7799846
GCGATCG6201.8189894E-1211.68581640
GTCGGAT8050.011.6136441
GTCGTAT8400.011.5419551
GTCGGTT6301.8189894E-1211.5419551
TCGACGC6301.8189894E-1211.49869243
GCCGTAT6401.8189894E-1211.3616111
CTATCGG4055.951879E-711.07289159
TTCGACC6653.6379788E-1210.89365230
GTCCGAT7450.010.6898641
TCTCGAC7750.010.68364241