FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_246_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_246_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4585774
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGAT5800.013.68060948
TAGCGCG1550.00811702313.35442349
CTCGATT5850.012.97395549
TCGATTC7750.012.46385750
CGATACG8500.010.96003629
GATTCCG8750.010.6451552
CCCGGAC8050.010.28348711
CGGTACA7403.6379788E-1210.25845161
TAAGCGC4754.197227E-710.165997520
ATTCCGT7503.6379788E-1210.11946453
TACGATA8550.010.09158827
GGCGGTA7302.7284841E-119.92618859
CGATTCC9550.09.7534151
TTCTCGA8405.456968E-129.44592947
GATACGG10600.09.43745930
CTAAGCG5301.8713545E-69.11103519
TCACCCG9500.09.0762883
TTAAACC16500.09.000742
GTAGTCG12000.08.91387657
GATATCG3100.00772332778.9004255