FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4585774
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG3000.020.69699731
CGACCGT3250.020.16630632
GCGATCG3650.017.95864740
CGATCGG3700.017.7157741
CCGATAT1452.7267545E-416.6534823
GTTCGAC3850.016.1275329
CGTGCGA4150.015.79443937
CCCGATA2201.6106478E-615.6934042
TTAATCG4000.015.5256248
TTCGACC4750.014.52420830
GATCGCG1709.1238663E-414.20568140
AGTTCGA4455.456968E-1213.95303128
TCGACGC4700.013.94539443
AAGTTCG4557.2759576E-1213.64637227
GTCGTTT7500.012.9345441
CGCGTAA2153.658499E-412.83688767
ATTCGAA5400.012.7782948
GACCGTC5800.012.48956733
CCGGTAT7300.012.3397051
GTCCCGA4852.8921932E-1012.1439931