FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_245_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_245_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12487312
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTA34600.08.9713047
TCACTAA38300.08.9150888
GTAGTCG13550.08.40245957
ACTCACT48200.08.3004546
AACTCAC47950.08.1998415
CGGTACA15100.07.768594761
CACTCTA36200.07.71974635
GTCGAAT5906.802459E-57.62020061
ACTCTAC37000.07.36646136
GTTTAAT56100.07.3359971
CACTAAC44700.07.25297989
CTCGATT13851.8189894E-127.223488349
AGTCGCC17000.07.10314959
CAACTCA55550.07.0158754
CGCTAGT5000.0064870696.899483733
CGAAAAT17350.06.7772661
GTCGATT7703.539885E-56.73714071
CTTTAAT64800.06.72466471
GGAATCG23700.06.69425343
TCGTTGA16500.06.690434551