FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12487312
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG4400.018.03238931
CGACCGT5600.014.16830532
GTCGGTT10750.013.8536171
GTCGCAT8400.013.193921
GTCCGTT16300.012.5362491
CCCGTAT9650.012.2026681
GTTCGAC7100.012.14630329
GTCGTAT14750.011.5055461
GTCGTTT20500.011.1504721
CCGGCTA11050.010.92770837
GTCCGGA17500.010.8849861
GTCGGAT18700.010.3716931
GTCCGAA12050.010.34711
GTCCGAT16200.010.2619381
TCGACGC9500.010.16713543
GTCGAAT11850.09.9371941
GTCCAGT59800.09.6141321
GGCGTAT14800.09.5945661
GTCGATA6909.840733E-109.5369111
TTCGACC9150.09.425302530