FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11014045
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA14100.07.58567161
GATTCCG12052.3646862E-117.44355752
GGCGGTA15600.07.298587359
GTTAGAT28600.07.25299261
CTCGATT10951.380613E-97.24625949
GTTAAAC34350.06.9447131
GTTTAAT51350.06.86738971
TCTCGAT13553.783498E-106.619727648
GTTCAAT29750.06.50778251
GCGGTAC18000.06.32547160
TTCTCGA13653.083187E-96.31843447
GTCTAAG18650.06.28901344
ATTTACG11157.433391E-76.18677856
TCGATTC14055.848051E-96.13843850
GTGAAAT85400.06.072471
GTCCGAA8601.6353242E-46.03010371
GTTACCG9802.4616336E-55.99728731
GTTTTAT83350.05.972951
GTTTGAT69000.05.962521
GTTTTTT136650.05.94552471