FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11014045
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT9350.014.4431791
GTCGGAT12700.012.2692671
CCCGTAT11200.012.0574761
GTCGTAA9150.011.7314161
GTCGGTA11250.011.6960951
GTCGGAA14200.011.4609171
GTCGCTT14900.011.387271
GTCGTTT19850.010.4664781
GTTCGAT15300.010.4106111
GTCCGGA14350.010.3759141
GTCCGTT13500.09.7467461
GCGATCG7504.7293724E-119.66066840
CCCGATT8650.09.6073791
GGCGTTT19200.09.5583841
GTCGTCT18650.09.4689361
TCGACCG6201.7922503E-89.45861331
CGACCGT6252.042907E-89.38294532
GTCGGCT18150.09.3482271
GTCGCAT7454.0199666E-109.2957311
GTTCGGA13350.09.0781351