FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_243_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_243_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12402424
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT13850.010.46188249
TCGCGAA3501.9071708E-49.85495720
TCGATTC15550.08.20867150
GATTCCG16550.08.129580552
TCTCGAT17600.07.84084548
GTTTAAT61150.07.7475381
ATTCCGT18850.07.320658753
CGGTACA18650.07.215582461
TTCTCGA19000.07.08143547
GGCGGTA17550.07.077864659
CGATTCC20800.06.63434751
GCGGTAC20800.06.46974160
GTTTATT79650.06.4256271
TTTCTCG23150.06.40763846
GTTTTTT167300.06.3664071
CGTCGTA10301.2537203E-66.363820651
TAAATCG8753.1630232E-56.30676463
GACGATC15502.0372681E-106.23235838
GTTAAAT58250.06.05541471
GTGAAAT98450.06.0416051