FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12402424
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGA16350.012.2867581
CCCGAAT12250.011.8751821
TCGACCG7100.011.66086431
GTCGTAT13800.011.5453171
GTCCGTT15400.011.4703471
GTCCGCT14550.010.712151
GTCGGAA16050.010.5742141
CCCGTAT10350.010.3740521
GTCGTTT21050.010.3661041
TCGCGTA4401.726974E-610.19307766
CTCGCGT6858.0035534E-1110.1126861
GTCGGTT11100.09.9851391
CGACCGT8450.09.79788632
CTCGTAT17350.09.582281
GTTCGAC9000.09.58226929
GCCGTAT10550.09.52078151
GTCCGTA15050.09.4357061
GTCCCGT22100.09.4034251
GTCGATT11950.09.2748991
CGCGTAA4502.4881698E-59.19994367