FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20939992
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT26150.09.49863349
GATTCCG30300.08.76675952
CGGTACA32850.08.71780961
GGCGGTA32900.08.49462959
TCTCGAT29650.08.37753848
TCGATTC32200.08.1423350
ATTCCGT30150.08.00943253
GCGGTAC41350.07.676741660
GTCTAAG41250.07.610361644
TTCTCGA32900.07.549843347
CGGGAAT35550.07.0040871
GTAGTCG16700.06.817933657
CGATTCC39550.06.803604651
TCACCCG41800.06.7659853
ACGGGCG46500.06.75214456
ACTAGGC24700.06.70267511
GCGTAGT17000.06.695825618
TAGCATA59700.06.64385379
GCGTCGT25000.06.622732619
GTTTTTT277300.06.60974361